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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which results in chromosome instability, and thus could lead to tumorigenesis. This protein was observed to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential job in endorsing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already described. Two extra splice variants were described but thorough nucleotide sequence facts is just not however available.
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Blocking peptides are peptides that bind especially to the focus on antibody and block antibody binding. These peptide commonly includes the epitope recognized with the antibody.
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This manual outlines even more possible optimization of cellular and immunoassay parameters to be certain the very best benefits are...
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This guidebook outlines additional feasible optimization of mobile and immunoassay parameters to be certain the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to the epitope about the target protein. By comparing the staining with the blocked antibody as opposed to the antibody by itself, you can see which staining is particular.
Concentrate on Details Cyclin E belongs towards the very conserved cyclin relatives, whose associates are characterized by a remarkable periodicity in protein abundance in the mobile cycle. Cyclins function as website regulators of CDK kinases. Various cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of every mitotic celebration. Cyclin E kinds a fancy with and features as a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates on the G1-S phase boundary which is degraded as cells development via S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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